What is Abbe Refractometer?

Abbe Refractometer is an optical instrument designed based on the principle of total reflection. It is mainly used to measure the refractive index and average dispersion of transparent or translucent liquids and solids. In many fields such as chemistry, biology, and medicine. Its principle is based on Snell's law, that is, when light enters from one medium into another, there is a certain proportional relationship between the angle of incidence and the angle of refraction. This proportional relationship determines the degree of refraction of light, thereby revealing the influence of matter on the propagation characteristics of light.

So how to use Abbe Refractometer? Make sure that the measuring platform and sample holder of the Abbe refractometer are clean and dust-free. Use a dropper to drop the sample to be tested on the sample holder to ensure that the sample is evenly distributed. Then place the sample holder on the measuring platform of the Abbe refractometer and close the lid.

Observe the scale in the eyepiece, move the focusing handwheel until the scale mark is clearly visible, and record the scale value at this time. Next, calculate the refractive index. According to the measured scale value and the temperature of the sample, use the attached refractive index calculation table to find the corresponding refractive index value. Note that the temperature of the sample should be as stable as possible at 20 degrees Celsius. Finally, use non-woven cloth or paper towel to gently wipe the measurement platform and sample holder of the Abbe refractometer to ensure they are dry and clean.